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seed pod

#1 User is offline   Yeong 

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Posted 05 August 2014 - 10:07 AM

I found this seed pod in my cattleya, likely to be pollinated by insect/bird.
Intend to harvest the seed pod and grow it at home (without using laminar flow), has anyone done it
successfully at home?

https://farm6.staticflickr.com/5594/14832244325_4907b6f96e_o.jpg

This post has been edited by Yeong: 05 August 2014 - 10:21 AM

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#2 User is offline   digitalgate 

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Posted 05 August 2014 - 11:15 AM

I saw a thread somewhere u might want to search for it .
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#3 User is offline   keff 

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Posted 05 August 2014 - 11:28 AM

There was also another thread where a forumer described an old time method of scattering the seeds near the roots of the parent plant in hope that the mycorrhizae there would associate with the seedling roots. Maybe you could try that too?

I wonder if those mychorrhizal pellets used for gardening would be of equivalent function? Or maybe the type of fungus is actually very specific?
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#4 User is offline   sthh 

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Posted 05 August 2014 - 01:38 PM

I asked this question before too.

You can try home flasking, and this is the thread i wrote about some time back.
Home Flasking

Or you can wait until your seed pod is ripe and bring it over to AVA for them to flask for you.
AVA
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#5 User is offline   Yeong 

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Posted 05 August 2014 - 02:35 PM

View PostYeong, on 05 August 2014 - 10:07 AM, said:

I found this seed pod in my cattleya, likely to be pollinated by insect/bird.
Intend to harvest the seed pod and grow it at home (without using laminar flow), has anyone done it
successfully at home?

https://farm6.staticflickr.com/5594/14832244325_4907b6f96e_o.jpg


I wii do home flasking. Let"s wait for the seed pod to mature.
Will let you know the outcome.
Thank you.
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#6 User is offline   Yeong 

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Posted 11 September 2014 - 07:53 PM

https://farm4.staticflickr.com/3872/15020309328_c9c10ef354_o.jpg

About 40 days old
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#7 User is offline   sthh 

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Posted 11 September 2014 - 08:00 PM

You may want to refer to this website to know how long it takes for cattleya seed pod to mature.

Harvesting Times of Orchid Seed Capsules for the Green Pod Culture Process
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#8 User is offline   AhSeng 

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Posted 12 September 2014 - 12:32 PM

My plant's pod turn yellow at 4 1/2 month.
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#9 User is offline   Yeong 

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Posted 12 September 2014 - 04:27 PM

Thank you for your info.
I usually based on observation of the seed pod, look at it closely after about 3 months and harvest it
when it strated to turn yellow, this will reduce the risk of seed pod bursting.
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#10 User is offline   Yeong 

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Posted 20 October 2014 - 10:32 AM

https://farm6.staticflickr.com/5613/15393199587_5df2a61cc9_o.jpg

Harvested the seed pod yesterday as it has shown hairline cracks.
The harvesting time is about 100 days.Will prepare medium and grow the seeds in flask.
Although I have done it in a commercial lab many years ago (was helping my relative's tissue culture
business), this is the first time I do it at home. Really not sure if it can be sucessful.
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#11 User is offline   Yeong 

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Posted 04 December 2014 - 10:48 AM

Managed to sow the seed in plastic containers without fugus problem (that is what I am
worry about).

https://farm9.static...5e5522717_o.jpg
35 days after sowing the seed. Germination has started and hopefully, protocorms (plant tissue)
can be developed soon.


https://farm8.static...3946f350d_o.jpg
20 days after sowing the seed.

https://farm9.static...e7b159412_o.jpg
I did it in a sterilised fish tank (spay lots of alcohol inside the tank and cover it and leave it for an hour)
in my kitchen.

https://farm8.static...c547220f9_o.jpg
The containers was placed near my kitchen window, with direct sunlight.

I have also prepared another batch using dessert containers. They are more transparent and I can see throught
the containers to inspect how the seed develop.
https://farm8.static...2082f77b9_o.jpg

I would like to thank sthh, digitalgate and keff for thier contributions and providing me
with useful info.
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#12 User is offline   sthh 

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Posted 04 December 2014 - 10:58 AM

Wow, exciting. Take more pictures along the way.
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#13 User is offline   keff 

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Posted 04 December 2014 - 08:03 PM

View Poststhh, on 04 December 2014 - 10:58 AM, said:

Wow, exciting. Take more pictures along the way.


Yes, please do keep us updated.

And wishing you lots of healthy baby plants!
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#14 User is offline   tc68 

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Posted 04 December 2014 - 08:15 PM

Congrats and good luck!
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#15 User is offline   Yeong 

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Posted 05 December 2014 - 11:53 AM

Thanks to all.
Hope protocorms can be developed, will have to wait. But not all seeds can form protocorms, if that happens, only
"fiber" is formed, no real plant tissue.

I will pollinate another seed pod, then harvest it when 85% ripe and sow the seed directly to a
agar medium withou sterilising it with sodium hypochlorie solution.This way will inceases the chance of
establishing protocorms from seeds. (Sometime, the sodium hypochlorite solution used to sterilse the seeds
can "kill" the seeds.)
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#16 User is offline   sthh 

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Posted 05 December 2014 - 12:27 PM

View PostYeong, on 05 December 2014 - 11:53 AM, said:

Thanks to all.
Hope protocorms can be developed, will have to wait. But not all seeds can form protocorms, if that happens, only
"fiber" is formed, no real plant tissue.

I will pollinate another seed pod, then harvest it when 85% ripe and sow the seed directly to a
agar medium withou sterilising it with sodium hypochlorie solution.This way will inceases the chance of
establishing protocorms from seeds. (Sometime, the sodium hypochlorite solution used to sterilse the seeds
can "kill" the seeds.)


Is sodium hypochlorie solution bleach fumes?
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#17 User is offline   Yeong 

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Posted 05 December 2014 - 02:57 PM

Yes, sodium hypochlorite is known as bleach. It has strong disinfection power.
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#18 User is offline   Yeong 

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Posted 02 March 2015 - 10:24 AM

Not successful. Protocorms doesn't develop.
Anyway, I have pollinated 2 seed pods. Will harvest them at about 85% ripe and sow the seed
directly without sterilising with sodium hypochloride solution.

https://farm9.static...b0a3537d9_o.jpg
https://farm9.static...0640a4739_o.jpg

This post has been edited by Yeong: 02 March 2015 - 10:26 AM

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#19 User is offline   AhSeng 

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Posted 02 March 2015 - 10:54 PM

yes, use green pod culture is easier than using seeds that have been exposed to the air.

This post has been edited by AhSeng: 02 March 2015 - 11:04 PM

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#20 User is offline   Yeong 

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Posted 13 November 2015 - 12:24 PM

Sorry for the delay, as I was working on different types of containers for this Home
method that will mininise contamination rate (reduce fugus grow).

I have found that using glass bottle from an Instant Coffee works quite well. Do not
used disposible plastic container as it gives high contamination rate.

The following photo shows germination from a green pod culture, 2.5 months after sowing the seed.
https://c1.staticfli...d19dd9893_n.jpg
https://c1.staticfli...5253b698c_n.jpg

Glass bottle from instant coffee:
https://c2.staticfli...27eed18cf_n.jpg

As it is a home method, many thins have to be modified. We can't follow what the commercial lab does. All items used
at home have are easily availble and they are not expensive.
I will give a detailed procedures (step by step) of this Home Method in Green Pod culture (both gel
preparation and seed sowing) in my next post. Allow me to have some time to organise.
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