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Ti§h's CP - 16 Nov : Scarification Tracking my plant growth from start

#1 User is offline   tish 

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Posted 30 December 2012 - 02:19 PM

I intend to keep track my plant growth in this local forum. Will keep updating very frequently.Previously I had purchased some mix VFT seeds from unknows source from ebay. I have also bought 2 more packet of VFT seeds from "Royal red" and "Saw Tooth UK #1" cultivars from a German guy, hopping they will grow up with similar traits to their parent.Photos of the package/ seeds -VFT Seeds bought from Ebay advertise as "Mixed VFT Seeds"http://sphotos-g.ak.fbcdn.net/hphotos-ak-snc6/224117_10151155219347297_1175910389_n.jpg
They are really small using a ruler to measure, approx 1mm
http://farm9.staticflickr.com/8343/8205259651_9acb9b1e78_o.jpg

Randomly picked 3 seeds intended to sow
http://farm9.staticflickr.com/8063/8205259383_aa69cf97a6_o.jpg

Water Germination : Using a transparent container and distill water with sterilized cottonThis is the first batch and I call them Mixed Batch, started 21th Nov 2012
http://farm9.staticflickr.com/8488/8205259105_1b1e26bfe0_o.jpg

The Packet of various CP along with 2 other VFT cultivars' seeds
http://sphotos-g.ak.fbcdn.net/hphotos-ak-ash4/463809_10151158986877297_1037884506_o.jpg

The other VFT seeds look almost exactly the same
http://farm9.staticflickr.com/8349/8216508983_7d06e22101_b.jpg

Some Sundew seeds that came together in the batch
http://farm9.staticflickr.com/8349/8217593596_f640e3940a_b.jpghttp://farm9.staticflickr.com/8337/8217593810_fb4da96f35_b.jpg

Drosera anglica

http://farm9.staticflickr.com/8477/8217593296_81ed98af90_b.jpg

Drosera rotundifolia
http://farm9.staticflickr.com/8339/8217594096_c97f1bc361_b.jpg

This post has been edited by tish: 16 November 2015 - 12:44 AM

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#2 User is offline   tish 

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Posted 30 December 2012 - 02:31 PM

Edited: I forgot to indicate that I have attempt water germination in 3 different batch at this point.

Mixed Batch, sow 21 Nov 2012 : Ebay seller, mixtures of different VFT Cultivar seeds (10 seeds)

RR DR Batch, sow 25 Nov 2012: German seller from forum, 2 Royal Red VFT seed, 3 Drosera Rotundifolia seeds

ST DA Batch, sow 25 Nov 2012: German seller from forum, 2 Sawtooth UK#1 VFT seed, 3 Drosera Anglica seeds


First Germination!

I'm quite happy, when I took up the mixed batch to take a good look, I realized one of the seed had something pointing out of it's tip. I took my Camera and snap on a magnification lens and I see 1 seed had germinated. This is my first experience on VFT and water germinating them.

Mixed batch Day 10: 1 seed germinated in distill water
http://farm9.staticflickr.com/8070/8234525870_a8f951f993_h.jpg

Here's a closed up
http://farm9.staticflickr.com/8338/8233462807_b984ae66d1_h.jpg


Few days later,


Previously some seeds were showing signs of germination, but I couldnt confirm as there wasnt much light at night. the following moring I went to inspect each of the VFT seed, what looks like to me are the early stage of germination. But later I've found out they were some kinda failed germinating, could be fungus or mold


Some of the seeds from the 3 batches
http://farm9.staticflickr.com/8486/8236042011_4f96503ff4_b.jpg


Here a seed from the ST DA Batch
Day 7
http://farm9.staticflickr.com/8204/8236042927_96b4d54131_h.jpghttp://farm9.staticflickr.com/8349/8236042713_75a16f3982_k.jpg

2 Seeds from RR DR Batch
Day 7
http://farm9.staticflickr.com/8344/8236042357_6297024028_h.jpg


Another one from RR DR Batch
http://farm9.staticflickr.com/8067/8237108680_97fb41a042_h.jpg

And Day 11 from the Mixed Batch, the same one I've posted earlier yesterday has grown so much over a night
http://farm9.staticflickr.com/8490/8237108306_2e637a451a_h.jpg

This post has been edited by tish: 10 January 2013 - 11:26 AM

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#3 User is offline   tish 

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Posted 30 December 2012 - 02:37 PM

A few days later, the seeds were confirmed not germinating, they must be mold or fungus, familed germinatingprogressed was the sole seed from Mixed batch.

Day 17
http://farm9.staticflickr.com/8199/8253289591_2673c3a342_h.jpg



As I was trying to figure out why was the tip of the VFT so hairy and pinkish. Little did I guess it was actually the roots of the VFT, now it kinda make sense that the roots would be the first to develop since water is 1 of the main source for it's growth. The tip that push the seed shell out were leaf.

BTW: It was a bad idea to use sterilized cotton as a backdrop for my photo taking, they tangled up with the roots so I had problem removing the ones in the roots, I had to leave a small portion in there. Not to mention I had some trouble untangling them from the leaves too.

Day 23: Mixed Batch sole survival of all 3 batches.

HELLO WORLD! I AM "FITTEST" thats what I'll name this VFT. @ Day 23
http://farm9.staticflickr.com/8499/8272798348_11a9ddc73f_k.jpg
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#4 User is offline   tish 

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Posted 30 December 2012 - 02:45 PM

Made "Fittest" into a time lapse video, focus point has been moving around due to water evaporating and refilling, seed growth etc. Hope you'll enjoy it.






https://vimeo.com/55704361
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#5 User is offline   tish 

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Posted 30 December 2012 - 02:54 PM

I've finally potted "Fittest" into a temporary take-away sauce container, 100% New Zeland Sphagnum moss, modified water tray method and plastic wrap with holes to minimize water evaporation. I hope I am doing it right.

"Fittest" sitting in a temporary container.
http://sphotos-h.ak.fbcdn.net/hphotos-ak-ash3/528314_10151187034637297_764006141_n.jpg


It's weekend and I had some free time to myself. The picture below is stacked with a total of 102 pictures in different focus point of the VFT, when stacked together - resulting in an "all area" clear focus photo. The area of focus is targeted on the plant only. The transparent "traps" (i think) is too small to be in clear focus. But I'll try to get a clear one when I have time again. Please forgive for the harsh photo, it was due to stacking.

Photo stack of "Fittest" @ Day 24
http://farm9.staticflickr.com/8216/8276213765_336eaab97e_o.jpg



Few Days Later,

After moving it to LFS, it starts to take some burns on the tip of the leave, probably it was due to a sudden change from 100% water to moist/dam moss. I'm not sure, but I hope it don't dry furthe.

Day 30, "Fittest" taking in some burns..
http://farm9.staticflickr.com/8081/8293460624_82301f9f05_o.jpg

That's a staple to give the estimation of the size.
http://sphotos-c.ak.fbcdn.net/hphotos-ak-ash3/156223_10151195887702297_1364335630_n.jpg



The next morning, I went to take a close look today, the second set leaves are already forming! I'm very happy. :D/> I'll be keeping watch on "Fittest"

Day 31, "Fittest" is growing 2nd set of leaves
http://farm9.staticflickr.com/8218/8296174849_bb58b58801_o.jpg

Here's a close up
http://farm9.staticflickr.com/8074/8296174503_28e8dd1221_o.jpg
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#6 User is offline   tish 

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Posted 30 December 2012 - 02:54 PM

I was reading about Tissue culture and thought about dropping some seeds in to agar-agar. I didn't put much effort in sterilizing as I think seeds should be quite easy to germinate in these media.

In-Vitro seed germination

TC Batch - VFT and Sundew seeds 21 Dec 2012
http://sphotos-h.ak.fbcdn.net/hphotos-ak-ash4/461625_10151196147417297_687944603_o.jpg

Christmas!
I''m almost at home all day during Christmas! Anyway to kill my boredom, I took out my camera gears and increase my camera's setup to maximum magnification.

Here's RR DR batch's "Santa" I named it that because I've observed it's germination on eve of X'mas http://www.greenculturesg.com/forum/public/style_emoticons/default/smile.gif

"Santa" at Day 30, germinated much later compared to "Fittest"
http://farm9.staticflickr.com/8215/8306442798_c0e9868e29_o.jpg

This post has been edited by tish: 10 January 2013 - 10:49 AM

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#7 User is offline   tish 

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Posted 30 December 2012 - 03:06 PM

One week later, I've observed some blackish stuff in the media, I could only suspect bacterial, I did not want it to kill my seedlings.

Bacterial / fungus / mold suspected
http://sphotos-f.ak.fbcdn.net/hphotos-ak-ash3/620953_10151207505972297_676725180_o.jpg

I quickly make plans to replate them. I use a baby bottle sterilizer and distill water to sterilze my used glass container. Re-sterilize seeds, transfer stuff as fast as I could . My pincer was wiped with Alcohol swipes. Its the best I could with everything I have.

TC VFT Batch and TC SD Batch - replated 28 Dec 2012
Keeping fingers cross
http://sphotos-h.ak.fbcdn.net/hphotos-ak-ash3/735799_10151207506162297_1255389838_o.jpg
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#8 User is offline   tish 

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Posted 30 December 2012 - 03:08 PM

Some Random updates some time later:

ST DA Batch: Nothing happens except the fungus head remains even after the last time I'd sterilized it. I Sterilized them again today so to remove the fungus head.

RR DR Batch: Lost the DR seeds somehow, out of the 2 VFT seeds, only "Santa" is continue growing, the other one is keeping still.

"Santa" at Day 33
http://farm9.staticflickr.com/8080/8316250669_91bac35937_o.jpg

Mixed Batch: out of 3 VFT seeds, only "fittest" is doing very well in moss now. The other 2, nothing happen to 1, how ever the other one that has a crack before I sow it remember? A small piece has chip off while I remove it for photo taking. What's inside is kinda funny, it looked exactly like the germinated seeds roots but only difference is totally pale white.

Mix batch before sow
http://farm9.staticflickr.com/8063/8205259383_aa69cf97a6_o.jpg

The chipped off seed
http://farm9.staticflickr.com/8073/8316250465_184373f7c2_o.jpg

Day 37 "Fittest". Sorry, i didnt realized I did not have a clear picture today
http://farm9.staticflickr.com/8495/8318388272_850d838083_o.jpg

This post has been edited by tish: 10 January 2013 - 10:51 AM

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#9 User is offline   tish 

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Posted 30 December 2012 - 03:09 PM

Last night I was looking into 1 of my old batch and observed another germination. It was a Drosera anglica seed. I was wondering why I had not noticed it a few days before while I was doing a lot of work on all my batches.

ST DA Batch : Drosera Anglica at Day 35
Naming it "Caterpillar" coz it's green and looked a little like this hehe
http://farm9.staticflickr.com/8081/8323348965_fa09ca535a_o.jpg

I think the pinkish part is the roots growth, just like the VFT
http://farm9.staticflickr.com/8351/8323349241_d6d36b378b_o.jpg

Another angle
http://farm9.staticflickr.com/8495/8324407458_6dcdeea877_o.jpg

This post has been edited by tish: 10 January 2013 - 10:52 AM

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#10 User is offline   Phantom 

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Posted 30 December 2012 - 04:41 PM

WOW! Your daily records are very detail. Nice macro shots too. Can you share how you prepare the vitro medium. Is it just from plain agar powder or did you add and ingredients to make it more nutritious? Hopefully, your experiments can determined which medium is best use for germinating carnivorous plants. Agar, Cotton or LFS. Good Luck growing!

By the way, it should be "Sawtooth UK1" not "sawtooth UKI" you read wrongly perhaps.
Dionaea muscipula "Sawtooth UK #1" is the full name

This post has been edited by Phantom: 30 December 2012 - 04:43 PM

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#11 User is offline   Clickclock 

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Posted 30 December 2012 - 07:42 PM

Really awesome photos! thanks for showing us, Its really interesting to see how VFT seedlings look like, they do look really delicate and small, seems tough to handle.
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#12 User is offline   tish 

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Posted 30 December 2012 - 09:37 PM

View PostPhantom, on 30 December 2012 - 04:41 PM, said:

WOW! Your daily records are very detail. Nice macro shots too. Can you share how you prepare the vitro medium. Is it just from plain agar powder or did you add and ingredients to make it more nutritious? Hopefully, your experiments can determined which medium is best use for germinating carnivorous plants. Agar, Cotton or LFS. Good Luck growing!

By the way, it should be "Sawtooth UK1" not "sawtooth UKI" you read wrongly perhaps.
Dionaea muscipula "Sawtooth UK #1" is the full name


Hi Phantom, Thanks for pointing out the mistake in the VFT name. Will change it. as for in-vitro medium, will share with you how I prepare the second time as the first time was very improper.

For seeds it is not so important for the overall procedure, the most important is not to get bacterial / germs into the medium, I admit my method is not done "cleanly" but still I've tried my best.

Approx 100ml of distill water
a pinch of sugar
a drop of food colouring
2 used glass bottles
a pinch of agar-agar powder (supermarket available agar-agar powder)

Pour the mixed together and divide into 2 glass container, gently rest the cap on top and set the 2 container in baby bottle sterilizer and run 1 time.

Rub the seeds in 10% Bleach and rinse the seeds in distill water, use pincer with alcohol cleaned and transfer the seeds ASAP into the "already gelled medium" container and capped tight.

I intend to release the cap without lifting up for awhile every 3 days for release of gasses, this is my second attempt and I'm not sure if it works. I did not use any other nutrients solution as I don't think seeds really need them.

Hope you find this info useful http://www.greenculturesg.com/forum/public/style_emoticons/default/smile.gif


View PostClickclock, on 30 December 2012 - 07:42 PM, said:

Really awesome photos! thanks for showing us, Its really interesting to see how VFT seedlings look like, they do look really delicate and small, seems tough to handle.


Hi Clickclock,

thanks http://www.greenculturesg.com/forum/public/style_emoticons/default/smile.gif My hobby happens to be macro-photography, but I am no expert. I use a pincer to handle the VFT seeds, they are quite hard actually, but I still take caution of not over holding it too tight.

This post has been edited by tish: 30 December 2012 - 09:37 PM

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#13 User is offline   tish 

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Posted 01 January 2013 - 12:45 PM

Happy New Year to all!!

I've got some updates here, some good, some bad. Well, start off with the bad news.

For 1 of my in vitro batch TC VFT. I've observed 2 seeds has grown mold/fungus on the tip of the seeds. I have removed them to re-sterilize and kept them separate container. Hopefully the other 2 VFT seeds stays and germinate. I also wonder, Why does all VFT seeds start mold/fungus on the tip of the seed.

TC VFT replated 28 dec 2012, mold/fungus observed
http://farm9.staticflickr.com/8073/8331403631_51a981ed93_o.jpg
http://farm9.staticflickr.com/8493/8332460610_f21a04489f_o.jpg



Drosera anglica from ST DA batch. Some fibres, I've assumed were root hairs has grown out of the base of the root top where the area is pinkish. The difference in growth over 2 days is quite a lot, even for VFT seedlings too. This is because I've been observing them in magnified views.


ST DA batch: "Caterpillar" Drosera Anglica Day 37
2 angles
http://farm9.staticflickr.com/8217/8332459934_cee1092826_o.jpg
http://farm9.staticflickr.com/8224/8331403383_8f6af9aa92_o.jpg



And the last update for today is my 2 VFT that has been growing a lot. I'm still waiting for "Fittest to form it's traps but seems like I have to wait longer. I've also noticed that "Santa's" been more reddish compared to "Fittest" in it's earlier stage. I'm hoping to see some reddish traits here. This is getting very exciting.


Mixed Batch Day 41 "Fittest", stacked with 50 photos to have more clear focus areas.
http://farm9.staticflickr.com/8079/8332463216_a0de0a8acd_o.jpg


RR DR Batch - Royal Red seed Day 37
"Santa" has more reddish/pinkish area coming too

http://farm9.staticflickr.com/8083/8331403041_acf8915d88_o.jpg

This post has been edited by tish: 10 January 2013 - 10:53 AM

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#14 User is offline   tish 

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Posted 05 January 2013 - 01:32 AM

Some updates, nothing much on "Santa and Fittest". They just grow longer. All the others VFT has no progress.

TC VFT Batch seeds has all been removed due to mold observed at the tip of the reminding 2 seeds.

"Santa" at Day 40
http://farm9.staticflickr.com/8358/8346770132_fcc2e388e5_o.jpg

"Fittest" a Day 44

http://farm9.staticflickr.com/8504/8345716089_911f772e37_o.jpg


As for the Sundews, TC Sundew has no mold observe, hopefully they stay that way. Some updates for for ST DA Batch.

Day 40 "Caterpillar" has grown spots!! I'm afraid it might just start moving and eating leaves, haha kidding.
This is an interesting sight. I only hope I had the process of spots appearing capture on motion pictures.
http://farm9.staticflickr.com/8502/8346769844_1807b25d4f_o.jpg

Another with a millimetre measurement
Approx 1mm in size, if you notice the husk is protecting a seed pod inside, the seed pod is about 0.5mm
http://farm9.staticflickr.com/8212/8346769626_1a1d5b04ac_o.jpg


Another Drosera Anglica germinated! Now in total, 2 out of 3 Sundew from ST DA Batch has germinated.

Naming it "Tiny" Day 40
http://farm9.staticflickr.com/8493/8345714717_f98aaebd6b_o.jpg

This post has been edited by tish: 10 January 2013 - 10:55 AM

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#15 User is offline   tish 

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Posted 09 January 2013 - 10:14 PM

Some minor updates:

No change to all the sleeping VFT seeds. "Tiny", my 2nd germinated Drosera Anglica was moved to a newly prepared TC Medium, its alone and after like almost a week, no sign of contamination.

TC Sundew batch has no obvious contamination but some seeds looked like has germinated, it's all blurry and I could not get a good shot, so I wont post any photo.


Day 49, "Fittest's" Trap has grow a little, but isn't going to open up any time yet as it's still 30% developed I think.
http://farm9.staticflickr.com/8373/8363660691_8410698513_k.jpg

Day 45, "Santa's" been getting quite big, its really making a lot of difference comparing with "Fittest" in the seedling days Look!
http://farm9.staticflickr.com/8238/8363653865_aa4ce04453_o.jpg

"Fittest" seedling picture, day 10 after sow.
Germinated approx 10 days later
Developed few roots hairs approx 2 days later
Fully develop first 2 leafs approx 11 days later (13 Days)
http://farm9.staticflickr.com/8490/8237108306_31d9a3843d_b.jpg


"Santa" A seed from Royal Red cultivar
Germinated approx 29 days later after sow
16 days later, no roots hair observed yet.(16 days and still no full first 2 leafs yet)
Growth rate is very different from "Fittest" from Mix batch, different thickness, different rate. I wonder why? "Fittest" is a seed from a mixtures of different cultivar VFT from Indonesia, "Santa" is from Royal Red, from Germany.

Another thing I suspected was, "Santa's" root could be in the seed pod and leaf were the ones that popped out first. Well, we'll see.

"Santa"!! Day 45
http://farm9.staticflickr.com/8238/8364720438_91383f2480_o.jpg


As for "Caterpillar", it's greenish color has drained a little, I'm getting a little worried, although it has grow a little, I've decided to pot it into a very tiny container, with 100% LFS , distill water in a small water tray. So we wont be seeing "Caterpillar" and "Tiny" for a while.

"Caterpillar" last photo shoot from water
http://farm9.staticflickr.com/8501/8364720604_85a78c1a23_o.jpg

This post has been edited by tish: 10 January 2013 - 10:56 AM

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#16 User is offline   tish 

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Posted 09 January 2013 - 11:28 PM

edited: a mistake i made thinking that I made a mistake. Everything was correct from day 1.




If you seen what I have edited, I have revert back to original




sorry for making everyone very confuse, I was confused! lol

This post has been edited by tish: 10 January 2013 - 11:00 AM

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#17 User is offline   tish 

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Posted 13 January 2013 - 12:54 PM

Hi all,

As the days goes, the Sundew cannot be observed using my camera's lens. So for now there is only photo update on the 2 VFT which are growing. I hope the remainder seeds germinate soon so there is more post and personal celebration.

The sundew germinated seeds remain in the potting and agar. It should be easier to observe when there is obvious growth in the potting, but the agar container holding TC Sundew batch and "Tiny" cannot be seen clearly.

There is some observation on "Fittest". Looks like the traps are taking shape slowly, I also think the cilia are forming from bottom upwards.

"Santa"!! Full or surprises!
Another surprise took place while observing a few hours ago. I have been thinking why is "Santa's" development so different from "Fittest". What I saw earlier had me more confuse. When "Santa" first germinate, I thought it is going to be normal just like any other Typical VFT especially "Fittest", some time last month, "Santa" don't look like roots growth, I was some how sure leaves were going to form. Today, I don't know what am I looking at. Callus is forming?? The process is still "Water Germination" Some enlightenment would be great here.

Mixed Batch - "Fittest" Day 53, Cilia forming
http://farm9.staticflickr.com/8193/8374539507_75d942c208_o.jpg

RR DR Batch - Royal Red seed "Santa" forming callus?
http://farm9.staticflickr.com/8043/8375613752_8bd695bf01_o.jpg
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#18 User is offline   tish 

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Posted 17 January 2013 - 12:16 AM

Hi all, just a quick update on "Fittest" It's big Trap has more tiny buds of cilia popping out, can't wait to observe more grow!

So I said "It's big trap" So you know there is 1 small trap already up haha.. Now I know they dont grow trap together. I guess they grow their trap one by one. When one has it's trap 30% formed, it's ready to throw out another. At least that's what I've gathered.

"Fittest" Day 56, More cilia on big trap and second trap is coming along on the far bottom left..
http://farm9.staticflickr.com/8192/8387138098_a3e4c8865d_o.jpg
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#19 User is offline   tish 

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Posted 18 January 2013 - 09:36 PM

I bought a 1 month old crown cutting ceph! and since Cephs are sensitive plants, I'll name it "Baby"

Sorry I got more different CP plant involve in my suppose VFT tracking thread. Here's a quick post.

I've asked around in this forum and someone is willing to sell me 1 of the many pots of crown cutting Cephalotus he's been growing since a month ago. I didn't get to see all the pots but he claim this one has the most roots. Well I don't really know Cephs as much as VFT for now. So If anyone could offer some advise, please do so.

This is a typical ceph, potted in 100% NZ dried Sphagnum moss, as mentioned above, crown cutting.


Here's a mobile phone photo of "Baby". A month old from a crown cutting of a 10 months old parent plant.
http://farm9.staticflickr.com/8473/8392266136_cd63ee0c06_o.jpg

This post has been edited by tish: 18 January 2013 - 09:38 PM

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#20 User is offline   tish 

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Posted 20 January 2013 - 12:51 PM

It's been a while since I've seen "Santa" upclose, last night I took some photos of "Santa" and here I am now updating. "Santa's" pretty deformed, the part where it grew what I thought was callus don't look like callus to me now. Coz I think when callus grows, they grow out a bunch together. I ran out of knowledge and I cannot imagine anymore. Just let "Santa" take it's own form...

"Santa" Day 55, looks like a worm with horns lol...
http://farm9.staticflickr.com/8049/8394372155_e5998a4a17_o.jpg

On the side
http://farm9.staticflickr.com/8053/8395456250_67c0ab2983_o.jpg

and a little closer
http://farm9.staticflickr.com/8051/8395456874_92f0c224fb_o.jpg



"Fittest's" been notti! As the big trap was lying on it's side, there's more development which was hidden away from my macro lens. Probably I wasn't observant enough.

I've been trying very hard to get a good overall photo on the entire trap in macro and failed at first. My lens aperture's been set to the smallest and I still cannot get a good wide focus. I resort to stacking technique again with 93 photos in different focus of the trap and stacked them to get a overall clear focus of the trap in a single photo.


"Fittest" at Day 60(sowed).
A partial developed/opened trap. Approx. 50 days after germination.
http://farm9.staticflickr.com/8469/8396323339_398456c2ae_o.jpg


Here's another stacked photo of "Fittest" from last night Day 59, Photo's a bit harsh.
http://farm9.staticflickr.com/8361/8395456646_12994e1f02_o.jpg


Cephalotus
It's time to water "Baby" and I was thinking why not re-pot and later water it so I wont disturb "Baby" too much. I used a 4+ inch styrofoam cup, made many holes at the bottom, tiny holes on the side so water can easily run through in case they slowed down in the middle. I boiled 100% NZ dried sphagnum moss with very mild fungicide in hoping to prevent fungus attack and root rot. Trying not to disturb the original soil of my ceph, I filled the cup with moss, transfer the original soil with ceph on the half filled cup, then filled the empty side with more moss.


Here's "Baby" in it's new pot, "Day 2" in my care
http://farm9.staticflickr.com/8193/8395465382_2ca1d95125_o.jpg


http://sphotos-d.ak.fbcdn.net/hphotos-ak-ash3/150093_10151243190087297_130434130_o.jpg
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