| |
THE
METHOD
 |
Here
is the flask used for this demonstration. It is Paphiopedilum
niveum var semi-alba.
Try
not to pull off the dead parts as you may rip the delicate
seedling apart - use a clean sterilized pair of scissors
instead.
|
|
Open
up the flask to aerate it for a day. This is to enable the
flasked plants to acclimatize with the external environment.
Some
choose to skip this step but I am doing this as it is more
of a habit.
On
the other hand, in our humid and hot environment, the agar
medium may be more prone to contamination when left opened
for too long.
|
 |
 |
Peeping
into the flask, note the thick furry roots, this is a sign
of well grown seedlings in flasks.
Unfortunately,
due to my delay, the bottom leaves have started browning
off due to stagnation of the flask medium after too long.
Note
that unlike the parent plants, the mottling of seedlings
is not usually as clear as those that are out of flask for
some time.
|
|
Line
your work surface with newspaper to catch any debris for
ease of cleaning up later.
Lay
another 3 to 4 sheets of newspaper in the centre.
|
 |
 |
The
purpose of those extra pieces of newspaper is to wrap up the
opened flask. |
| Wrap
the flask tightly in the extra sheets of newspaper and hold
the ends firmly with one hand. |
 |
|

|
With
the free hand, use either a pair of pliers or a hammer to
knock the bottom of the flask.
Some
flasks are specially designed to have a bottom rim that
is thinner than the rest of the flask, resulting in a clean
break.
Some
growers wouldl use a glass cutter to score the bottom rim
before breaking.
|
| When
a clean cracking sound is heard, the flask would been broken
sufficiently. |
 |
 |
This
demonstrates a clean break where the top of the flask separates
nicely from the bottom rim.
Be
careful of any fine glass shards that occur.
Carefully
remove the glass from the seedlings and agar.
|
|
If
you intend to grow the seedlings in a community pot, do
not ease the seedlings apart but simply wash off the agar.
As
I am not particularly fond of community pots and personally
advocate thumb pots as far as possible, I carefully separated
out the seedlings.
Be
careful when separating the seedling of Brachys as they
have a very fragile root junction (between the plant tip
and start of the roots), despite having very thick strong
roots.
|
 |
|
|
|